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. 2022 Nov 3;17(11):e0275258. doi: 10.1371/journal.pone.0275258

Fig 8. Leonurine decreased ROS production by flow cytometric detection in H9c2 cells stimulated by TGF-β.

Fig 8

DCFH-DA was used to evaluate the intracellular ROS level. DCFH-DA is a nonfluorescent ester of the dye fluorescent product in the presence of ROS. (A) The peak value of the model group shifted to the right, and the peak value of the leonurine group shifted to the left. (B) ROS production increased with TGF-β exposure, and pretreatment with leonurine inhibited the ROS increase after TGF-β stimulation. ##P<0.01, model group vs. control group; **P<0.01, 20 μM leonurine group vs. model group. DCFH-DA, 2,7-dichlorofluorescein diacetate.