Skip to main content
. 2022 Nov 3;11:e79525. doi: 10.7554/eLife.79525

Figure 6. Regulators of the three GAPDH isoenzymes Tdh1, Thd2, and Tdh3.

Figure 6.

(A) Pairwise comparison of effects of gene perturbations on Tdh1, Tdh2, and Tdh3 protein abundances. Only genes that upon perturbation affect both Tdh proteins significantly are shown (FDR<0.05). (B) Ras/cAMP/PKA pathway representation with components colored if they showed a significant signal for one or more of the GAPDH isoenzymes (red: activators, blue: inhibitors). The scheme in (B) has been adapted from Figure 1 from Peeters et al., 2017, with added Mck1 (Griffioen et al., 2003; Peeters et al., 2017). (C) Heatmap representing all genes involved in the PKA pathway that upon perturbation affect at least one of the three GAPDH isoenzymes significantly (FDR<0.1). (D) Effects of IRA2 W342* and RAS2 Q272* mutations on Tdh1 and Tdh2 protein abundance observed in the base editor screen (‘GFP’) and by LC-MS-based proteomics on the individual mutants ('MS'). (E) Effects of IRA2 W342*, RAS2 Q272* and RAS2 S46L mutations on Tdh1, Tdh2, and Tdh3 protein abundance observed in the base editor screen. The heatmap color scale is identical for C, D, and E, and is capped at –1 and 1.