Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain and strain background (chikungunya virus [CHIKV]) | CHIKV viral replicon particles (VRPs) | DOI:10.1186/1743–422X-10-235 | The viral genome is split into three RNAs, as described in the DOI, resulting in VRPs that cause a single-cycle infection. | |
| Strain and strain background (Escherichia coli) | BL21(DE3) | In-house stock | Used for protein expression. | |
| Strain and strain background (E. coli) | XL10 | In-house stock | Used for cloning. | |
| Cell line: (Mesocricetus auratus) |
Baby hamster kidney (BHK)-21 (C-13) | ATCC | Cat: CCL-10 | Fibroblast cell line. |
| Commercial assay and kit | LookOut Mycoplasma PCR detection kit | Sigma-Aldrich | Cat: MP0035 | Outcome was negative throughout this work. |
| Recombinant DNA reagent | CHIKV nsP1 E. coli expression plasmid | This paper | Cloned for CHIKV nsP1 protein expression. Synthetic gene based on GenBank: KT449801.1. Available upon request. | |
| Recombinant DNA reagent | CHIKV nsP2 E. coli expression plasmid | This paper | Cloned for CHIKV nsP2 protein expression. Synthetic gene based on GenBank: KT449801.1. Available upon request. | |
| Chemical compound and drug | Protease Inhibitor cocktail | In-house preparation | Benzamidine: Sigma-Aldrich Cat: B6506-25G PMSF: VWR Cat: 0754–5 G Leupeptin: Alfa Aesar Cat: 15483809 |
|
| Chemical compound and drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) | Avanti Polar lipids | Cat: 850457 C | |
| Chemical compound and drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) | Avanti Polar lipids | Cat: 840034 C | |
| Chemical compound and drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (POPG) | Avanti Polar lipids | Cat: 840457 C | |
| Chemical compound and drug | Phosphatidylinositol (PI) | Avanti Polar lipids | Cat: 840042 C | |
| Chemical compound and drug | PI(4)P | Avanti Polar lipids | Cat: 840045 P | |
| Chemical compound and drug | PI(4,5)P2 | Avanti Polar lipids | Cat: 840046 P | |
| Chemical compound and drug | Cholesterol | Avanti Polar lipids | Cat: 700100 P | |
| Chemical compound and drug | ATTO-647N-DOPE | Sigma-Aldrich | Cat: 42247–1 MG | Manufactured by ATTO-TEC. |
| Chemical compound and drug | ATTO- 488 NHS |
Invitrogen | Cat: 41698–1 MG-F | Manufactured by ATTO-TEC. |
| Software and algorithm | ImageLab | Bio-Rad | Used for gel quatification. | |
| Software and algorithm | Prism | Graph-Pad | Used for generation of plots. | |
| Software and algorithm | ImageJ | NIH | Confocal images were processed using ImageJ. | |
| Software and algorithm | DiscoverMP software | Refeyn Ltd | For mass photometry data processing. | |
| Software and algorithm | SerialEM | DOI:10.1016/j.jsb.2005.07.007 | Data acquisition for cryo-electron tomography. | |
| Software and algorithm | MotionCor2 | DOI:10.1038/nmeth.4193 | Motion correction of cryo-ET data. | |
| Software and algorithm | CTFFIND4 | DOI:10.1016/j.jsb.2015.08.008 | CTF (contrast transfer function) correction of cryo-ET data. | |
| Software and algorithm | IMOD | DOI:10.1006/jsbi.1996.0013 | Reconstruction of cryo-electron tomograms. | |
| Software and algorithm | Amira | Thermo Fisher Scientific | Segmentation of cryo-electron tomograms and filament tracing. | |
| Software and algorithm | Dynamo | DOI:10.1016/j.jsb.2011.12.017 | Subtomogram averaging. | |
| Software and algorithm | MATLAB | Mathworks, Inc. | General image processing and running Dynamo. | |
| Software and algorithm | UCSF chimera | DOI:10.1002/jcc.20084 | Visualization and segmentation of subtomogram averages. |