Fig. 2.

Immune responses after immunization with LeCoVax-2 vaccine. (A-D) Antibody responses in sera of immunized mice were evaluated. (A) Variation of IgG levels detected at days 21, 35 and 48 and IgA levels at day 48 (B) after SC or R administration of different vaccine formulations in mice (PBS administered as control; n = 10 mice per experimental group, except for PBS-R, n = 5). Dots represent original data points, diamonds represent estimated mean values (with 95% CI) from a gamma GLMM accounting for heterogeneity of variances (see Methods). Different letters denote significant (p < 0.05) differences at post-hoc tests (after Tukey correction for multiple testing) within each time point (for IgA determination upon rectal administration, no pairwise comparison was significant after correction for multiple testing). (C) Neutralizing antibody titers at day 48 post-administration of vaccines (left: subcutaneous; right: rectal; note the different y-axis scales). Bars represent median values, error bars 25th and 75th percentiles. Random jittering was added to datapoint to show overlapping data. The horizontal dashed line represents the threshold over which samples were considered as the limit of detection (LOD). Antibody titers were determined only for samples that were IgG positive at day 48 post-administration. The PBS-SC group was included as a control for both subcutaneous and rectal administration; see Methods).