Fig. 3.

Evaluation of antigen-specific cytokine-producing T cells capacity. The number of IFN-γ, IL-4 and TNF-α secreting cells per million splenocytes (spot forming units, SFU) was determined by ELISpot analysis at day 48 post-administration of LeCoVax-2 through the SC route (A) and R route (B). Only samples that were IgG positive at day 48 post-administration were evaluated. Bars represent median values, error bars 25th and 75th percentiles. For rectal administration, we lacked PBS treated individuals (see Methods): for comparison, we thus reported the values obtained from the single individual which received a SC injection.