Fig. 4.

Histological analyses for adverse effects determination. (A) Total number of lymphoid structures, GALT total score and total area of lymphoid structures in the intestinal tract of R-vaccinated mice in proximity of the ileo-cecal-colic junction are shown. Box plots represent median, 25th and 75th percentiles, minimum and maximum values. Arrows indicate lymphoid structures of gut-associated lymphoid tissue. (B) Body weight variation of mice subjected to subcutaneous (upper panel) or rectal (lower panel) administration of different vaccine formulations (including PBS administration as a control group) in mice (n = 10 mice per experimental group, with the exception of PBS-R, n = 5). Dots represent original datapoints, diamonds represent estimated mean values (with 95% CI) from a LMM (see Methods). No pairwise comparison (within each time point) was significant upon correction for multiple testing.