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. 2022 Oct 20;10:1050808. doi: 10.3389/fbioe.2022.1050808

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Copyright © 2022 Zheng, Guo, Zhang, Liu, Fan and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Synthesis of D-allose by fermentation using E. coli (DGI, DAE, RPI, GalP). E. coli (DGI, DAE, RPI, GalP) was cultured in M9 medium with D-glucose and glycerol at 30°C. (A) Recombinant plasmids of pETDuet-galP-dgi and pRSFDuet-dae-rpi. (B) D-Glucose, D-fructose, D-allulose, and D-allose. (C) Glycerol and cell density. Error bars indicated standard error (n = 3).