FIGURE 6.

ICAM1‐mediated promotion of TNBC bone metastasis was associated with TGF‐β1 and EMT. Representative images of IHC staining of ICAM1 (A), integrin β2 (B), TGF‐β1 (C), and N‐cadherin (D) in bone metastases in TNBC tumor‐bearing mice in the three groups. E, Violin plot of the mean gray values in the bone metastases, as calculated with ImageJ (N = 3). *p < 0.05, **p < 0.01 vs. the Par group; ^# p < 0.05, ^## p < 0.01 vs. the BM group. EMT, epithelial‐to‐mesenchymal transition; IHC, immunohistochemical; ICAM1, intercellular adhesion molecule 1; TNBC, triple‐negative breast cancer