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. 2022 Feb 8;40(4):423–434. doi: 10.1093/stmcls/sxac006

Figure 2.

Figure 2.

Nuclear actin increases after dynamic strain. Nuclear YFP-NLS-βActin in MSC increases after dynamic, but not static strain (3 examples of each condition shown, scale bar = 25 μm (A). Western blot of cytoplasmic and nuclear lysates (B) was quantified by densitometry n = 4, P < .005 (C). IP nuclear protein using anti-GFP and IB YFP and β-catenin shows association between the 2 molecules (D). Cell cytoplasmic and nuclear lysates were IP for IgG, actin or βCat and immunoblotted for actin (E). AFM-measured modulus of isolated nuclei showed that dynamic strain increased the nuclear modulus by 2-fold (P < .001) compared to control and 1.3-fold compared with static strain groups (P < .001). Static strain increased nuclear modulus by 1.5-fold compared with control (P < .05) (F).