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YAP1, but not β-catenin, responds to generation of cytoplasmic F-actin. Mesenchymal stem cells at 10 000 cells/cm2 were treated with LPA (50 μM) for 24 hours and stained for YAP1 (red), nucleus (yellow), and F-actin (green) (A) or βCat (yellow), nucleus (blue), and F-actin (green), scale bar = 25 μm (A, C). Nuclear/cytoplasmic density of YAP1 or β-catenin IF was quantitated by ImageJ, ∗ = P < .0001 (B, D).
