Fig. 3. Metastatic capacity of DNMT3B^H tumor cells compared with DNMT3B^L cells.

A. DNMT3B, DNMT1, and DNMT4A Western blot for DNMT3B^H and DNMT3B^L clonal 4T1 cell lines. B. Surface lung metastases (met) counts from mice that received MFP injections of DNMT3B^H, DNMT3B^L, and DNMT3B^H-KD 4T1 cells. C. Schematic experimental design for metastatic potential analysis of DNMT3B^H, DNMT3B^L, DNMT3B^H-KD 4T1 cells. D. Quantitation of DNMT3B^H-GFP or DNMT3B^L-RFP lung metastases at week 4 from mice that received MFP injection of tumor cells (n=5) (left panel). Representative images are on the right. The white dotted lines indicate the lung boundary. E. Flow cytometry analysis of GFP⁺ or RFP⁺ tumor cells at week 4 from the lungs of mice that received MFP injection of tumor cells (left panel), with representative plots of GFP⁺ or RFP⁺ tumor cells (right panel). F. DNMT3B^H and DNMT3B^L metastases in the lungs in a time course experiment (2, 3, and 4 weeks, MFP injection) in which the DNMT3B^H and DNMT3B^L cells were injected together for clonal competition. Upper left, schematic design; Lower left, quantitative data; Right panels, representative IF images. Quantifications of band density are presented in Western blot. Data are presented as mean ± SD. ** p < 0.01, *** p < 0.001.