Table 4.
Primers
| Gene | Forward primer | Reverse primer |
|---|---|---|
|
| ||
| BARX1 | CCAGTGGGAACTTGAACACC | CTGAAGTTCGGCGTGCAG |
| COL1A1 | GACGAAGACATCCCACCAATCA | GGACTCGTCACAGATCACGTC |
| CYP26A1 | GCTGCCTCTCTAACCTGCAC | TGCTTTAGTGCCTGCATGTC |
| DES | AATGACCGCTTCGCCAACTA | GGTTAGTGAGCACCTCCACC |
| FOXA2 | GGGAGCGGTGAAGATGGA | TCATGTTGCTCACGGAGGAGTA |
| FOXF1 | AGCAGCCGTATCTGCACCAGAA | CTCCTTTCGGTCACACATGCTG |
| GATA4 | TAGCCCCACAGTTGACACAC | GTCCTGCACAGCCTGCC |
| GLI1 | GGGATGATCCCACATCCTCAGTC | CTGGAGCAGCCCCCCCAGT |
| HAND2 | CACTTCTGAATTCGCCCAC | TCTCTTTGACCCCCTTTGAG |
| HOXA5 | CGCCCAACCCCAGATCTAC | CGGGCCGCCTATGTTGT |
| ISL1 | AGATTATATCAGGTTGTACGGGATCA | ACACAGCGGAAACACTCGAT |
| KRT19 | CACCAGCCGGACTGAAGAAT | GCAGGTCAGTAACCTCGGAC |
| LHX2 | TCGGGACTTGGTTTATCACCT | GCAAGCGGCAGTAGACCAG |
| MSC | TATGAGAACGGCTACGTGCAC | AGTCCGATTTAAGCGGTGGTT |
| MSX1 | CTCCGCAAACACAAGACGAAC | GGCGGTTCTGGAACCATATCT |
| MSX2 | CGCCAAGACATATGAGCCCT | GTTCTGCCTCCTGCAGTCTT |
| NKX2–5 | TGGAGAAGACAGAGGCGGACAA | ATAGACCTGCGCCTGCGAGAA |
| NKX3–2 | CAACACCGTCGTCCTCG | CCGCTTCCAAAGACCTAGAG |
| NKX6–1 | ATGACAGAGAGTCAGGTCAAGG | CTCCGAGTCCTGCTTCTTCTT |
| OCT3/4 | TGAGAGGCAACCTGGAGAATT | TTTCTTTCCCTAGCTCCTCCC |
| PITX1 | TTCTTGGCTGGGTCGTCT | TCGTCTGACACGGAGCTG |
| PTCH1 | CCACAGAAGCGCTCCTACA | CTGTAATTTCGCCCCTTCC |
| SOX2 | GCTTAGCCTCGTCGATGAAC | AACCCCAAGATGCACAACTC |
| SOX9 | GTAATCCGGGTGGTCCTTCT | GTACCCGCACTTGCACAAC |
| TBX4 | TGATCATCACTAAGGCTGGCAG | ACAGAACTTGTAGCGATGGTCAT |
| TBX5 | ACAAAGTGAAGGTGACGGGCCTTA | ATCTGTGATCGTCGGCAGGTACAA |
| TBX18 | TTAACCTTGTCCGTCTGCCTGAGT | GTAATGGGCTTTGGCCTTTGCACT |
| TBX20 | GGCGACGGAGAACACAATCAA | CTGGGCACAGGACGACTTC |
| TBXT | CAGTGGCAGTCTCAGGTTAAGAAGGA | CGCTACTGCAGGTGTGAGCAA |
| UPK1B | TGGAAGCAACGAACAGTTGA | CTACCGTGTGCGCAGAAA |
| UPK3B | ATACCGTCTGGCTCGTGGTG | CAGGGGCAGCGTCATGTAGT |
| VIM | ATTCCACTTTGCGTTCAAGG | CTTCAGAGAGAGGAAGCCGA |
| WNT2 | CTGTATCAGGGACCGAGAGG | CCCACAGCACATGACTTCAC |
| WT1 | ATAGGCCAGGGCATGTGTATGTGT | AGTTGCCTGGCAGAACTACATCCT |