FIGURE 1.
BCL-XL is a Strong Dependency in a Subset of Kidney-lineage Cancer Cells. A, List of dependencies enriched for selectivity in a subset of kidney lineage cells, as indicated by Likelihood Ratio Test (LRT) scores > 100. B, Comparison of BCL-XL dependency in the RNAi versus CRISPR/Cas9 dependency maps, in the indicated kidney cancer cell lines. Negative scores indicate a dependency with scores < −1 (strong, green oval), −0.5 to −1.0 (intermediate, blue oval), and >−0.5 (neutral/resistant, red triangle), annotated based on the CRISPR/Cas9 dataset. Immunoblots (C) and crystal violet staining (D) of the indicated cell lines that were lentivirally transduced to express shRNAs targeting the indicated BCL-2-family genes or non-targeting controls (Con). Immunoblots (E) and crystal violet staining (F and G) of the indicated cell lines that were lentivirally transduced to express the indicated shRNA-resistant versions of BCL-XL or GFP, as a control, followed by lentiviral expression of the indicated shRNAs. (C) and (E) were done 3 days post infection with the shRNA-expressing lentiviral particles. (D), (F), and (G) were done 3 days post selection for A-498, CAKI-2, and UMRC-6 cells, and 7–10 days post selection for UMRC-2 cells.