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. Author manuscript; available in PMC: 2022 Nov 4.
Published in final edited form as: Nat Protoc. 2022 Jul 13;17(11):2415–2430. doi: 10.1038/s41596-022-00714-6

Fig. 4 |. Precursor chromatogram for positive-mode iRT calibrants.

Fig. 4 |

The overlayed extracted precursor lipid iRT calibrant chromatograms for positive ionization mode from a test plasma sample are displayed. This is viewed in Skyline by selecting the ‘Positive Plasma Lipid iRT Calibrants’ molecule list name in the ‘Targets’ pane. Tip: Holding the shift key and pressing the down-arrow key advances the selection through the peptides in the list, highlighting each in red on the overlayed chromatogram graph.