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In vitro characterization of ADP‐ribosylation of rifamycins using overexpressed Arr Mab . All the peaks were identified by MS (Table S5). The retention time difference of the peaks is labelled. Rifabutin was fully converted into the ADP‐ribosyl adduct upon incubation in a 40‐minute time course. By contrast, no Arr Mab ‐catalyzed transformation was observed for the synthetic compound 5 a under the same incubation condition.
