Figure 2. Bile acids regulate ENaC Po in split open mouse distal renal tubules.
A, Distal renal tubules were isolated from mice and subjected to cell-attached patch using pipettes backfilled with either 1 mM t-CA or 1 mM t-HDCA. B, Representative continuous current traces with −Vp = −60 mV, during which bile acids diffused towards the tip of the pipette. Inward currents are downward, and the number of open channels is indicated by dashed lines. Po was estimated from the indicated time spans representing the control and diffused conditions. C, Effect of bile acids on ENaC Po. Po determined from control and diffused conditions were compared using paired Student’s t-test. Individual experiments (blue; n = 13 collecting ducts for t-CA; n = 11 collecting ducts for t-HDCA) and summary statistics (mean (SD)) are shown. Created with BioRender.com