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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: J Physiol. 2022 Sep 23;600(21):4695–4711. doi: 10.1113/JP283318

Figure 9. Amiloride does not reduce DCA-p crosslinking to ENaC.

Figure 9.

FRT cells expressing ENaC subunits with a single subunit bearing a C-terminal V5 epitope tag were treated with 50 μM DCA-p ± 100 μM amiloride, exposed to UV light, and processed to isolate probe-labeled proteins. Input, unbound and eluted fractions were separated by SDS-PAGE followed by immunoblot with an anti-V5 antibody. A, Representative immunoblot. B, Quantified densities of input and eluted proteins were used to calculate the fraction of crosslinked ENaC. Individual data points (colored circles) and summary statistics (mean (SD)) are shown. The number of replicates (culture wells) are indicated in parentheses. Data were analyzed by two-way ANOVA with Šidáks multiple comparison test.