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. 2022 Nov 3;13:6427. doi: 10.1038/s41467-022-33797-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Validation spectra for the unique peptides used to detect C4A (a), C4B (c) and total C4 (tC4) (e) in human cerebrospinal fluid (CSF). b, d, f display linear range of heavy peptides in pooled CSF digest for C4A, C4B, and tC4, respectively. Spiked pooled CSF samples were analyzed three times at each concentration. Each data point on the graph represents the mean of the three values. Source data are provided in the Source Data file.