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. 2022 Nov 3;5:1174. doi: 10.1038/s42003-022-04139-5

Fig. 3. Knockout mutations spanning the DNMT3B protein have minimal effects on proliferation across AML cell lines wild-type and mutant for DNMT3A.

Fig. 3

a–c sgRNAs tiling DNMT3B in AML cell lines wild-type for DNMT3A, NOMO-1 (a), OCI-M1 (b), and THP-1 (c) are shown with respect to their cDNA position along the coding region of DNMT3B. d–f sgRNAs tiling DNMT3B in AML cell lines mutant for DNMT3A, OCI-AML2 (d), SIG-M5 (e), and OCI-AML3 (f) are shown with respect to their cDNA position along the coding region of DNMT3B. Each circle represents an individual sgRNA targeting DNMT3B. The standardized LFC value for each sgRNA is plotted on each graph. The dashed black bar denotes the mean standardized LFC of the non-targeting controls for each cell line. The mean standardized LFC of the common essential sgRNAs is denoted by the red dashed line. The color of each circle represents the functional domain it targets: PWWP (light gray); ADD (black); MTase domain (blue). n = 3 replicates for each cell line screened.