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. 2022 Oct 21;16:969058. doi: 10.3389/fncel.2022.969058

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Copyright © 2022 Lisi, Marinelli, Ciotti, Pizzoferrato, Palmerio, Chiavari, Cattaneo and Navarra.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects on urea pathway after 48 h nerve growth factor (NGF) treatments in CHME-5. (A,B) The effects of 1–10 or 100 ng/ml of NGF on urea production under basal condition and in co-stimulation with IL4 respectively. (C,D) The effects of 1–10 or 100 ng/ml of painless human Nerve Growth Factor (hNGFp) on urea production under basal condition and in co-stimulation with IL4 respectively. *p < 0.05 and ****p < 0.0001 vs. IL4. One-way ANOVA analysis followed by Bonferroni’s post-test was carried out. (A) F(5, 30) = 16.54; P < 0.0001. (B) F(3, 19) = 6.368; P = 0.0036. Data are expressed as mean ± SEM. The experiment was repeated four times with similar results.