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. 2022 Oct 21;16:969058. doi: 10.3389/fncel.2022.969058

TABLE 1.

Antibody information used for western immunoblotting analysis.

Antibody Dilution Producer Incubation time and temperature conditions
anti p- AKT ser 473 XP 1:1000 Cell signaling 2 h, room temperature
anti tot AKT 1:1000 Thermo Fisher Scientific 2 h, room temperature
anti p- Creb 1:1000 Cell signaling overnight, 4°C
anti β-actin 1:1000 Sigma 2 h, room temperature
anti p-MEK 1/2 1:1000 Cell signaling overnight, 4°C
anti total MEK 1/2 1:1000 Cell signaling overnight, 4°C
anti IkB α 1:1000 Santa Cruz 2 h, room temperature
Anti-mouse 1:3000 Sigma 1 h, room temperature
Anti-rabbit 1:15000 Jackson ImmunoResearch 1 h, room temperature