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. 2022 Nov 3;13:6320. doi: 10.1038/s41467-022-33796-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Extracellular flux analysis (Seahorse XF Cell Mito Stress kit) was used to assess the effects of VWF-binding upon macrophage metabolism. Extracellular acidification (ECAR) was measured to study the effects on glycolysis following stimulation with VWF (10 μg/ml) (blue), LPS (100 ng/ml) (red), or untreated controls (black) for 3 h (a, b) and 16 h (c, d) respectively. Similarly, cellular oxygen consumption rate (OCR) was assayed to study the effects on BMDM oxidative phosphorylation after 3 h and 16 h (e, f) incubations. The effects of VWF and LPS on BMDM glycolysis (*P = 0.0272 and ***P = 0.0004 for control vs LPS; *P = 0.0279 and P = 0.0646 for control vs VWF, following 3 and 16 h respectively) and oxidative phosphorylation were studied in the presence or absence of specific mitochondrial inhibitors. Plots are representative images collected from three independent assays. The data are presented as the mean values ± SD for three independent experiments. Significance was determined by ANOVA in which **p < 0.01 and ****p < 0.0001. Source data for this figure are provided as a Source Data file.