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. 2022 Nov 3;13:6320. doi: 10.1038/s41467-022-33796-7

Fig. 6. VWF influences mitochondrial dynamics and up-regulates HIF-1α expression.

Fig. 6

a, b Murine BMDMs were incubated in the presence or absence of pd-VWF (10 μg/ml) or LPS (100 ng/ml) for 3 or 16 h and mitochondrial morphology assessed using Mitotracker and scanning confocal live cell imaging as detailed in the Materials and Methods. A minimum of 20 images including ≥60 mitochondria per cell were analyzed per treatment. Following 3 h stimulation with either VWF or LPS, a significant increase in mitochondrial fragmentation consistent with an increase in glycolysis was observed (p < 0.01 and p < 0.001 respectively). Although a significant increase in mitochondrial fragmentation was still observed following a 16 h incubation with LPS (****P < 0.0001), it was no longer observed in BMDMs treated with VWF (P = 0.958, ns = not significant). c, d BMDMs were treated with either VWF (10 μg/ml) or LPS (100 ng/ml) for 3 or 16 h (****P < 0.0001 for control vs LPS and P = 0.3065 for control vs VWF at 16 h), and then HIF-1α or PHD3 expression were assessed using Western blotting or qRT-PCR respectively. The data are presented as mean values ± SD for three independent experiments. The significance was calculated by ANOVA where *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 respectively. Source data for this figure are provided as a Source Data file.