TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference(s) |
|---|---|---|
| N. gonorrhoeae strains | ||
| FA19 | LF+ TF+ | 5, 23 |
| FA1090 | LF− TF+ | 8 |
| FA6775 | LF− Cmr (lbpA::mTn3 Cm) | 3 |
| FA6815 | TF− Strr Spcr (tbpB::Ω) | 2 |
| FA6839 | LF− TF+ Strr Spcr (lbpB::Ω); transformant, pUNCH130→FA19 | This study |
| FA6965 | LF+ Kanr (lbpB::aphA3); transformant, pUNCH195→FA19 | This study |
| FA6985 | LF− (lbpA::mTn3 Cm) TF− (tbpB::Ω), Cmr Strr Spcr; transformant, FA6775→FA6815 | This study |
| FA6986 | LF+ (lbpB::aphA3) TF− (tbpB::Ω) Kanr Strr Spcr; transformant, FA6965→FA6815 | This study |
| Plasmids | ||
| pHP45Ω | Source for the Ω fragment (Strr Spcr) | 32 |
| pMCL210 | Cloning vector, low copy; lacZα Cmr | 27 |
| pUC18K | Source for the aphA3 cassette (Kanr) | 24 |
| pUNCH127 | Apr Tetr; 2.5-kb FA19 lbpAB SspI fragment cloned into SspI site in pBR322 | 3 |
| pUNCH128 | Apr; FA19 insert in pUNCH127 cloned into BamHI site in pUNCH615 | 3 |
| pUNCH130 | Strr Spcr; Ω cassette inserted into AvaI site in pUNCH128 | This study |
| pUNCH142 | Apr; 2.3-kb FA19 RsaI fragment cloned into BamHI site in pUNCH615 | This study |
| pUNCH192 | Like pUNCH192SB but unstable | |
| pUNCH192SB | Cmr; 1.1-kb FA19 Sau3A fragment cloned into BamHI site in pMCL210 | This study |
| pUNCH193 | Like pUNCH192SB but deleted | This study |
| pUNCH195 | Kanr; aphA3 cassette inserted into HpaI site in pUNCH142 | This study |
| pUNCH615 | Apr; pBluescript II KS(−) with PstI site replaced with MluI | 32 |