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. 2022 Nov 3;13:6585. doi: 10.1038/s41467-022-34225-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Split-GFP strategy for K07H8.10 (NUCL-1) visualization in the germline. b NUCL-1 localizes to nucleoli in the adult germline. c Confocal-DIC imaging reveals that NUCL-1 localizes exclusively to the nucleolus in oocytes and pachytene germ cells. Pink arrows indicate nucleolar vacuoles. d NUCL-1 displays precise sub-nucleolar organization within oocytes (1 nucleolus shown) and pachytene germ cells (5 nucleoli shown). e NUCL-1 and FIB-1 occupy distinct spaces in pachytene germ cell nucleoli. f GARR-1::sGFP11 in pachytene germ cell nucleoli. g GARR-1 and FIB-1 occupy the same sub-nucleolar compartment in pachytene germ cells. h LPD-6::sGFP11 in pachytene germ cells. i A human nucleolus is organized into 3 phase-separated layers and ribosome biogenesis occurs in a step-by-step manner from the inside-out. j Yeast nucleoli are organized into 2 phase-separated compartments. k C. elegans nucleoli contain 2 phase-separated compartments, with NUCL-1 and LPD-6 in the GC and FIB-1 and GARR-1 in the FC. (FC fibrillar center, DFC dense fibrillar component, GC granular component, RNA Pol1 RNA polymerase 1).