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. 2022 Nov 3;13:6585. doi: 10.1038/s41467-022-34225-5

Fig. 4. The NUCL-1 RG/RGG domain contributes to sub-nucleolar organization in the germline and in the soma.

Fig. 4

a Pachytene germ cell nucleoli in NUCL-1 WT and a’ NUCL-1ΔRGG adult worms (4 nucleoli per image). b Single nucleoli in NUCL-1 WT and b’ NUCL-1ΔRGG pachytene germ cells. White dashed line represents area measured. NUCL-1 WT is enriched in the GC (blue arrows) and depleted in the FC and nucleolar vacuoles (pink arrows). c Histogram of fluorescence intensity for the nucleolus in b. NUCL-1 WT intensities show peaks corresponding to areas of enrichment (blue arrow) and depletion (pink arrow). c’ Histogram of fluorescence intensity for the nucleolus in b’. NUCL-1ΔRGG intensities group into a single peak. d NUCL-1ΔRGG CV is decreased compared to WT (n = 168 nucleoli from 12 NUCL-1 WT worms, 183 nucleoli from 12 NUCL-1ΔRGG worms). p = 2.67E-51. e No difference in nucleolar volume was detected in NUCL-1ΔRGG worms compared to WT (n = 100 nucleoli from 10 NUCL-1 WT worms and 91 nucleoli from 10 NUCL-1ΔRGG worms). f FIB-1::wrmScarlet and NUCL-1::sGFP11 in NUCL-1 WT and NUCL-1ΔRGG pachytene germ cell nucleoli. In NUCL-1 WT nucleoli, FIB-1 is in the FC (pink arrow) and NUCL-1 is in the GC (white arrows). FIB-1 in NUCL-1ΔRGG nucleoli shows no compartmentalization into the FC. g FIB-1 in NUCL-1ΔRGG nucleoli has decreased CV compared to WT (n = 111 nucleoli from 7 NUCL-1 WT worms and 118 nucleoli from 7 NUCL-1ΔRGG worms). p = 3.19E-16. h The volume of FIB-1-labeled nucleoli in NUCL-1ΔRGG worms is comparable to WT (n = 55 nucleoli from 7 NUCL-1 WT worms and 61 nucleoli from 7 NUCL-1ΔRGG worms). i FIB-1 in NUCL-1WT and NUCL-1ΔRGG hypodermal cells. 3 nucleoli shown per image. j Single FIB-1-labeled nucleoli in NUCL-1WT and NUCL-1ΔRGG hypodermal cells. k The CV for FIB-1 in NUCL-1ΔRGG hypodermal nucleoli is decreased compared to WT (n = 88 nucleoli from 10 NUCL-1 WT worms and 84 nucleoli from 11 NUCL-1ΔRGG worms). p = 2.89E-05. l The volume of FIB-1-labeled hypodermal cell nucleoli in NUCL-1ΔRGG worms is comparable to WT (n = 70 nucleoli from 9 NUCL-1 WT worms and 81 nucleoli from 10 NUCL-1ΔRGG worms). d, e, g, h, k, l Unpaired, two-tailed t test with Welch’s correction. Points on graphs represent individual nucleoli and means ± SD are shown.