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. 2022 Oct 19;25(11):105341. doi: 10.1016/j.isci.2022.105341
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Caspase 3 Cell Signaling CST9661
Caspase 9 Cell Signaling CST9502
GAPDH Cell Signaling CST2118
PARP Cell Signaling CST9532
PLK1 Abcam ab17056
phosphorylated PLK1 Abcam ab133442
anti-mouse HRP-conjugated secondary antibody Cell Signaling 7075
Anti-rabbit HRP-conjugated secondary antibody Cell Signaling 7074

Chemicals, peptides, and recombinant proteins

Nonidet P40 (NP40) containing protease and phosphatase inhibitors ThermoFisher Scientific 78446
Isocove’s Modified Dulbecco’s Medium ThermoFisher Scientific 12440054
Heat-inactivated fetal bovine serum Atlanta Biologicals S11150

Critical commercial assays

CellTiter-Glo Luminescent Cell Viability Assay Promega G7573
Apo-ONE homogeneous caspase-3/7 assay Promega G7792
LumiGLO Kit Cell Signaling 5067501

Deposited data

Processed data This paper https://github.com/LoLab-VU/MAGINE_Supplement_notebooks/blob/master/Data/bendamustine_data.csv.gz
https://doi.org/10.5281/zenodo.6639374

Experimental models: Cell lines

Human: HL-60 ATCC CCL-240

Software and algorithms

MAGINE This paper https://github.com/LoLab-VU/MAGINE
MAGINE docker image This paper docker pull lolab/magine-complete
MAGINE notebooks for this study This paper https://github.com/LoLab-VU/MAGINE_Supplement_notebooks