REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Caspase 3 | Cell Signaling | CST9661 |
Caspase 9 | Cell Signaling | CST9502 |
GAPDH | Cell Signaling | CST2118 |
PARP | Cell Signaling | CST9532 |
PLK1 | Abcam | ab17056 |
phosphorylated PLK1 | Abcam | ab133442 |
anti-mouse HRP-conjugated secondary antibody | Cell Signaling | 7075 |
Anti-rabbit HRP-conjugated secondary antibody | Cell Signaling | 7074 |
Chemicals, peptides, and recombinant proteins | ||
Nonidet P40 (NP40) containing protease and phosphatase inhibitors | ThermoFisher Scientific | 78446 |
Isocove’s Modified Dulbecco’s Medium | ThermoFisher Scientific | 12440054 |
Heat-inactivated fetal bovine serum | Atlanta Biologicals | S11150 |
Critical commercial assays | ||
CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7573 |
Apo-ONE homogeneous caspase-3/7 assay | Promega | G7792 |
LumiGLO Kit | Cell Signaling | 5067501 |
Deposited data | ||
Processed data | This paper |
https://github.com/LoLab-VU/MAGINE_Supplement_notebooks/blob/master/Data/bendamustine_data.csv.gz https://doi.org/10.5281/zenodo.6639374 |
Experimental models: Cell lines | ||
Human: HL-60 | ATCC | CCL-240 |
Software and algorithms | ||
MAGINE | This paper | https://github.com/LoLab-VU/MAGINE |
MAGINE docker image | This paper | docker pull lolab/magine-complete |
MAGINE notebooks for this study | This paper | https://github.com/LoLab-VU/MAGINE_Supplement_notebooks |