Table 2.
Correlation of ATP hydrolysis with downstream signaling.
| NLRP3 protein | ATP hydrolysis | IL-1β secretion | ASC specking |
|---|---|---|---|
| wt | 1.00 | 1.00 | 1.00 |
| R262W | 0.53 | 0.38 | 1.78 |
| K232A | 0.34 | 0.08 | 0.15 |
| T233S | 1.21 | 0.85 | 1.54 |
| D305A/E306A | 0.22 | 0.16 | 0.27 |
| R351G | 0.40 | 0.04 | 0.15 |
| R351T | 0.10 | 0.49 | 0.13 |
| H522R | 0.86 | 0.07 | 0.26 |
Mean values of IL-1β secretion levels of LPS-primed immortalized macrophages and ASC specking (speck/nuclei) determined in HeLa cells transfected with 100 ng of DNA and reconstituted with indicated NLRP3 variants in comparison to ATP turnover numbers of recombinantly expressed NLRP3 variants, all normalized to wild type levels.