FIG. 4.

M. tuberculosis Nox19 (Ftn [BfrB]) is a homolog of bacterial nonheme ferritins. Shown is a multiple sequence alignment of M. tuberculosis (Mt) Ftn (BfrB) (Nox19 [the sequence is based on the N-terminal amino acid sequence in Fig. 3 and the corresponding M. tuberculosis H37Rv genomic translated sequence, where it was termed BfrB subsequent to the completion of this work]) with the previously characterized eucaryotic-type nonheme ferritins from H. pylori (Hp Pfr), C. jejuni (Cj Cft), E. coli ferritin (Ec Ftn1 [also known as RsgA]), and additional homologs from the genomic E. coli (Ec Ftn2) and Haemophilus influenzae (Hi Ftn1 and Ftn2) databases. An asterisk indicates that the open reading frame corresponding to H. influenzae Ftn1 in the database has been truncated to match the start codon corresponding to other bacterial nonheme ferritins. Identical residues are boxed; additional similarities and conservative substitutions among bacterial nonheme ferritins are noticeable, but are not indicated.