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. 2022 Nov 3;5:1172. doi: 10.1038/s42003-022-04153-7

Fig. 2. Sumire-T-sapphire based FRET probe.

Fig. 2

a Diagram of vgCam construct. b Emission spectra of vgCam in the presence of 20 μM CaCl2 (blue) and 10 mM EGTA (black). Spectra data are average measurements of independently prepared samples (n = 3). c A series of pseudo color ratio images of vgCam and ATeam1.03. Scale bar: 40 μm. d Time course of fluorescence intensity ratio of vgCam and ATeam1.03 inside ROI1 (black) and ROI2 (blue) depicted in the top-left image of c. 100 μM histamine was added at time 0.