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. 2022 Nov 4;12(11):149. doi: 10.1038/s41408-022-00745-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Circus plot showing GATA-3 target genes and chromosome location in CTCL cell lines. Target gene names identified in Mac1 (green), MyLa CD4 + (red), H9 (black), respectively, are indicated with the dots shown. Venn diagram of GATA-3 target genes in each cell line is shown, and target genes shared across all three cell lines are shown in red. Target genes used for validation are also shown in green. B Dot plots of representative pathways enriched with GATA-3 target genes in CTCL are indicated. C GATA-3 ChIP and binding enrichment at selected GATA-3 target genes (ITK, CCR4, and c-MYC) is shown (at left) for the 3 CTCL cell lines indicated, and for malignant T cells obtained from Sezary syndrome patients (n = 4). Gene expression upon GATA-3 knockdown (at right) was determined by qRT-PCR for ITK (top) and C-MYC (bottom). CCR4 expression was determined by flow cytometry and ∆MFI reported. Percent input (%input) was used as Y-axis in binding enrichment. Fold change was normalized to total GAPDH levels. D Circus plot showing GATA-3 target genes and chromosome location in T-ALL cell lines. Target gene names identified in THP6 (blue), SUPT1 (red), MOLT4 (green) CRF-CEM (black), respectively, are indicated with the dots shown. Venn diagram of GATA-3 target genes in each cell line is shown, and target genes shared across all four cell lines are show in red. Target genes used for validation are also shown in green. E Dot plots of representative pathways enriched with GATA-3 target genes in T-ALL are indicated. F GATA-3 ChIP and binding enrichment at selected GATA-3 target genes (ITK and c-MYC) is shown (at left) for the 4 T-ALL cell lines indicated. Gene expression upon GATA-3 knockdown (at right) was determined by qRT-PCR for ITK (top) and C-MYC (bottom). Percent input (%input) was used as Y-axis in binding enrichment. Fold change was normalized to total GAPDH levels. Data are represented as mean ± s.e.m (standard error of the mean). *p < 0.05, **p < 0.01.