FIGURE 3.

Total neutron scattering from B. subtilis JEBS102 cells grown in 90% D₂O growth conditions, with or without FA restriction and supplementation using the procedures described as a function of D₂O content in the resuspension buffer. Total scattering was assessed as the Porod invariant, Q*, over the range from 0.009 Å −1 < q < 0.06 Å −1. This is compared to the estimated total scattering intensity I(0) based on a model (Nickels et al., 2017b) of estimated biomolecule content and scattering length density. The incorporation of hydrogenated FAs in the partially deuterated cells leads to the observed increase in scattered intensity of FA labeled cells at high fractions of D₂O in the resuspension buffer.