TABLE 3.
Input parameters for the headgroup portion of lamellar model (Tan et al., 2021) of cell membrane structure taken from the B. subtilis lipid extract model (Nickels et al., 2017a). This portion of the membrane is contrast matched to 85% D2O resuspension buffer, making the resulting fit insensitive to this part of the membrane structure but if water content equal to the extract is assumed, nW = 11.6, a headgroup thickness (DH) of 11.3 ± 0.2 Å can be inferred.
| Headgroups | Mole (%) | Vol. (Å3) | Vol (%) | B (fm) | ρ (fm/Å3) | MW (g/Mole) |
|---|---|---|---|---|---|---|
| PE | 54.2 | 317.8 | 66.3 | 185.93 | 0.59 | 269 |
| PG | 9.3 | 325.1 | 11.6 | 182.87 | 0.56 | 299 |
| CL | 3.2 | 498.4 | 6.1 | 264.05 | 0.53 | 505 |
| DAG | 31.3 | 151.8 | 18.3 | 88.20 | 0.58 | 146 |
| FFA | 2.0 | 44.1 | 0.3 | 24.90 | 0.56 | 28 |
| Average per 2-chain lipid | 258.9 | 150.6 | 0.58 | 228.5 |
PE, phosphoethanolamine; PG, phosphoglycerol; CL, cardiolipin; DAG, diacylglycerol; FFA, free fatty acid.