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. 2022 Aug 23;33(6):e75. doi: 10.3802/jgo.2022.33.e75

Fig. 3. IGF2BP1 overexpression reversed the effects of MCF2L-AS1 silencing on ovarian cancer cisplatin-resistance.

Fig. 3

Cisplatin-resistant cells were transfected with sh-NC + Oe-NC, sh-NC + Oe-IGF2BP1, sh-MCF2L-AS1 + Oe-NC and sh-MCF2L-AS1 + Oe-IGF2BP1. (A) Western blot was performed to evaluate IGF2BP1 level. (B) Cell proliferation was evaluated using CCK-8 assay. (C) Colony formation assay was performed to detect colony formation. (D) Cell apoptosis was evaluated by flow cytometry assay. (E) Bax, Bcl-2 and Cleaved Caspase-3 levels were assessed using western blot. The data were expressed as mean ± SD. All data were obtained from at least 3 replicate experiments.

CCK-8, cell counting kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IGF2BP1, insulin-like growth factor-2 mRNA binding protein 1; NC, normal control; Oe-, overexpression plasmid of; SD, standard deviation; sh-, short hairpin RNA of.

*p<0.05, †p<0.01, ‡p<0.001.