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. 2022 Oct 21;13:1000755. doi: 10.3389/fimmu.2022.1000755

Figure 3.

Figure 3

Reduced B-1a B cell compartment and TI immune response in IκBNS conditional knock-out mice. (A) Representative flow cytometry plots showing gating strategy for CD19+ B220lo CD5+ CD43+ B-1a cells in peritoneal cavity (PC) and blood (left panel). Numbers adjacent to gates indicate cell frequencies in the parent population. Frequencies of B-1a cells in peritoneal cavity and blood (right panel). Bars and error bars represent mean ± SD. (B) Natural serum IgM levels. Graph lines and error bars indicate mean ± SD. (C) Correlation between serum IgM levels and frequencies of B-1a cells in peritoneum. Data are representative of three independent experiments with 3-13 mice per group for each experiment. (D) Anti-NP IgM and IgG3 antibody production in serum from mice 7 days after i.p. immunization with 50 µg NP-Ficoll. Graph lines and error bars indicate mean ± SD. Data are representative of two independent experiments with 3-7 mice per group. **P ≤0.01, ***P ≤0.001, and ****P ≤0.0001.