Table 3.

Mutations in E. faecalis 16C51-T1 isolate were detected by whole-genome sequencing.

ref_gene_ID	NA mutations	AA mutations	ref_gene_product
16C51T1_GM000842	G213T	R70S	MarR family transcriptional regulator
16C51T1_GM001052	T848G	D282A	ethanolamine ammonia-lyase subunit EutC
16C51T1_GM001288	A449C	K149T	TetR family transcriptional regulator
16C51T1_GM001991	C605T	S201F	sigma 54-interacting transcriptional regulator
16C51T1_GM002261	T457C	K152E	helix-turn-helix domain-containing protein
16C51T1_GM002262	T109C	K36E	MerR family transcriptional regulator
16C51T1_GM002375	A65C	E21A	YhgE/Pip domain-containing protein
16C51T1_GM002628	A169G	N56D	DUF3850 domain-containing protein

E. faecalis 16C51 strain was serially subcultured in TSB containing licochalcone A. Mutations in the isolate T1 from 63 generations of 16C51 strain were detected by whole-genome sequencing. NA, nucleotide; AA, amino acid.