Table 1.
Primer Forward (F) and Reverse (R) Sequences.
| Genes | Sequence |
|---|---|
| 18sF | CATTCGAACGTCTGCCCTATC |
| 18sR | CCTGCTGCCTTCCTTGGA |
| GADPH F | TGACTCTACCCACGGCAAGTTCAA |
| GADPH R | ACGACATACTCAGCACCAGCATCA |
| BMP4 F | ACACAGGGACACACCAACCAT |
| BMP4 R | TGTGACCAGCTGTGTTCATCTTG |
| Noggin F | GCAGCTGCTTCAGTAAGCG |
| Noggin R | GCACTCGGAAATGATGGG |
| DKK1 F | TTGGAAGGGTGGGAATGTGAC |
| DKK1 R | ACCATTCTTCAGCATGTCAGAG |
| Cer1 F | CACCAAATTCACCACCACGC |
| Cer1 R | CGTCTTCACCATGCACTGAC |
| Wif1 F | TTGTACCTGTGGATCGACGC |
| Wif1 R | GGCTGGCATTCTTTGTTGGG |
| DKK2 F | ATGGGTTTTGTTGTGCTCGC |
| DKK2 R | CAGCCCATGTGAACCCTTCT |
| DKK3 F | GACACTCAGCACAAACTGCG |
| DKK3 R | TCTCCGTGTTGGTCTCGTTG |
| DKK4 F | GCGGAGGAAAACAGACCTCA |
| DKK4 R | AAATGGCGAGCACAGCAAAG |
| Sfrp1 F | ACGTGAGCTTCCAGTCGGAT |
| Sfrp1 R | CTTCTTGTAGCCCACGTTGTG |
| Sfrp2 F | ATCCTGGAGACAAAGAGCAAGACC |
| Sfrp2 R | TGACCAGATACGGAGCGTTGATG |
| Sfrp3 F | TCCGGGCTAAAGTTAAAGAGGT |
| Sfrp3 R | GACGGTATCCCTTGGAATGT |
| Sfrp4 F | TGAGGTCACAACTGTGGTCG |
| Sfrp4 R | ACACTGGCAGGAGGAATTGG |
| Sfrp5 F | TTGCCTCCTACTTGGACGAC |
| Sfrp5 R | CACCCGCATGGCTGGATATT |
| SMAD1 F | CCAAATGGCTAGTGGGTCTACA |
| SMAD1 R | GAAACACAGCAAAGTCCGACAC |
| CDK2 F | CTTTGCCGAAATGGTGACCC |
| CDK2 R | TAACTCCTGGCCAAACCACC |