Table 6.
Items | CON | TA | AFB1 | TA + AFB1 | SEM | P- value | ||
---|---|---|---|---|---|---|---|---|
AFB1 | TA | AFB1 × TA | ||||||
Day 21 | ||||||||
T-AOC (nmol/mgprot) | 207.24 | 235.86 | 184.09 | 180.90 | 5.96 | <0.001 | 0.211 | 0.120 |
CAT (U/mgprot) | 15.28 | 18.58 | 15.81 | 18.66 | 0.66 | 0.813 | 0.021 | 0.861 |
GST (U/mgprot) | 23.35 | 26.88 | 16.69 | 20.32 | 0.84 | <0.001 | 0.006 | 0.969 |
GSH-Px (U/mgprot) | 63.38 | 69.81 | 31.68 | 44.88 | 3.11 | <0.001 | 0.022 | 0.413 |
T-SOD (U/mgprot) | 1692.04b | 1885.59a | 1443.62c | 1413.18c | 38.77 | <0.001 | 0.103 | 0.027 |
MDA (nmol/mgprot) | 1.71 | 1.65 | 1.73 | 1.58 | 0.06 | 0.864 | 0.404 | 0.730 |
Day 42 | ||||||||
T-AOC (nmol/mgprot) | 143.34 | 147.58 | 125.06 | 126.11 | 3.78 | 0.008 | 0.713 | 0.824 |
CAT (U/mgprot) | 19.32 | 18.96 | 19.49 | 19.84 | 0.60 | 0.676 | 0.998 | 0.778 |
GST (U/mgprot) | 52.71 | 54.63 | 47.56 | 44.78 | 1.27 | 0.002 | 0.851 | 0.315 |
GSH-Px (U/mgprot) | 58.87 | 56.95 | 51.96 | 60.32 | 1.51 | 0.552 | 0.282 | 0.090 |
T-SOD (U/mgprot) | 1766.21 | 1723.15 | 1606.53 | 1802.90 | 37.69 | 0.595 | 0.310 | 0.117 |
MDA (nmol/mgprot) | 2.01 | 1.86 | 2.00 | 1.70 | 0.09 | 0.642 | 0.202 | 0.674 |
aEach mean represents 10 replications with 2 broilers per replication. CON, control diet; AFB1, 500 μg/kg aflatoxin B1 of feed; TA, 250 mg/kg tannic acid of feed; TA + AFB1, 250 mg/kg TA + 500 μg/kg AFB1.
b T-SOD, total superoxide dismutase; GSH-Px, glutathione peroxidase; GST, glutathione S-transferase; T-AOC, total antioxidant capacity; MDA, malondialdehyde.
a,b,cMeans in the same row with no common superscripts differ significantly (P < 0.05).