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. 2022 Oct 21;13:967021. doi: 10.3389/fmicb.2022.967021

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Copyright © 2022 Valenzuela, Norambuena, Morgante, García, Jiménez, Núñez, Fuentes and Pollak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viroscope.io. The Viroscope web application enables virus plant diagnostics using HTS data. It provides a user-interface to perform the Viroscope pipeline, and yields the VGAC (viral genome assembly coverage) metric as well as the presence of replicases to interpret the data and perform diagnosis. The process involves uploading HTS reads into the read library, selecting an analysis against a specific viral panel using user-inputted thresholds. This generates the analysis which is processed in real-time. The analysis then displays the results in plots that provide the metrics and suggests the diagnosis according to the selected thresholds, which is then confirmed by the user. The background image for the Viroscope.io web app was purchased at Shutterstock.com under a standard license.