Figure 4.

The effects of interferon-related CCL5, IFI27, IFIT1, IFIT3, IFITM3, and OAS1 proteins on WSN- and DkPen-type virus RdRP enzymes in the HEK-293 cells. The cells were grown in 24 well plates, and co-transfected with a protein coding-plasmid (30/well or 100 ng/well), and a certain amount of minireplicon plasmids [WSN: pCAGGS-PB2/W (3 ng/well), pCAGGS-PB1/W (3 ng/well), pCAGGS-PA/W (1 ng/well), pCAGGS-NP/W (10 ng/well), pHH21-vNS-luc (5 ng/well), pRL (2 ng/well), DkPen: pCAGGS-PB2/D (10 ng/well), pCAGGS-PB1/D (10 ng/well), pCAGGS-PA/D (3 ng/well), pCAGGS-NP/D (10 ng/well), pHH21-vNS-luc, (5 ng/well) pRL (2 ng/well)]. After 48 hours transfection, the cells were lysed, and reporter luciferase and Renilla luciferase activities were measured with luciferase assay kits