FIGURE 3.

Effect of PCAT14 deletion on endothelial angiogenic responses and activation. Human umbilical vein endothelial cells (HUVEC, Passage 3) were transfected with a GapmeR (50 nmol/L, 72 h) targeting PCAT14 (PCAT^KD) or a respective control (CTL). (A) Percentage of endothelial cell confluence for up to 48 h. (B) Representative brightfield images and quantification following a wound scratch assay at 0 and 6 hours. (C) Representative brightfield images and quantification of endothelial cell sprouts on a 3D spheroid assay. (D) Representative brightfield images and quantification of THP-1 monocytes adhered to endothelial cells. As a positive control, endothelial cells treated with Interleukin 1β (Il-1β, 10 ng/mL, 4 h). n=3-6 biological replicates per group, experiments performed at least 2 times. Scale bar = 500 μm, *p<0.05, **p<0.01, Student’s t-test paired, N.S: Not Significant; log₂FC: log₂ (Fold Change); p-adj: p-value adjusted.