Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 21;13:998059. doi: 10.3389/fimmu.2022.998059

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Cui, Gutierrez, Ariai, Öberg, Thörn, Gehrmann, Cloonan, Naessens and Olsson

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Rotenone induces mtROS production and impairment of monocyte-to-macrophage differentiation. (A) Surface expression of indicated markers analyzed on monocyte differentiated with M-CSF in the presence of rotenone (10nM-10µM) for 1 day. Each symbol represents an individual donor (n = 4-6). Data shown are pooled from three experiments. (B) Phagocytic capacity of monocytes differentiated with M-CSF in the presence of 10µM rotenone for 1 day, represented as pHrodo E.coli bioparticle gMFI. Each dot represents an individual donor (n = 6). Data shown are pooled from three experiments. (C) Quantification of mtROS production in monocytes differentiated with M-CSF and treated with rotenone 10µM for 24h (n = 6). Data are presented as MitoSOX gMFI. (D) FACS analysis of the percentage of viable monocytes (taken as Zombie Green negative cells) differentiated with M-CSF and treated with rotenone for 1 day. Each symbol represents an individual donor. (A-C) One-way ANOVA with Dunnett’s multiple comparisons test was performed. ns, not significant, *p < 0.05, **p < 0.01.