Figure 2.

Transcriptional expression pattern analysis of GmMATE13. (A) Time course of GmMATE13 expression patterns in root tips under aluminum stress. After 7 days, the seedlings (Jiyu70 and Jiyu62) were transplanted to 0.5 mM CaCl₂ solution plus 30 µM AlCl₃ at pH 4.5. The 0-1 cm root tips were excised after 0, 2, 4, 8, 12, and 24 h Al exposure to investigate the expression of GmMATE13. (B) Effect of different metals on the expression of GmMATE13 in root apices. Seedlings were treated to 30 µM Al, 1 µM Cu, 10 µM La, or 25 µM Cd for 4 h, excising the 0-1 cm root tips. (C) Transcriptional expression of GmMATE13 with -Fe solution. The seedlings were cultivated in nutrient solution with or without Fe-EDTA for 10-day culture, excising the 0-1 cm root apices. Data are represented as means ± SD (n=3). Error bars with different letters represented significantly different by Tukey’s test (P<.05).