Table 1. qRT-PCR primer sequences.
Gene | Primer | Sequence (5′–3′) |
---|---|---|
Nlrp3 | Forward | CCCTTGGAGACACAGGACTC |
Reverse | GAGGCTGCAGTTGTCTAATTCC | |
Casp1 | Forward | ACCCTCAAGTTTTGCCCTTT |
Reverse | GATCCTCCAGCAGCAACTTC | |
Gsdmd | Forward | CCATCGGCCTTTGAGAAAGTG |
Reverse | ACACATGAATAACGGGGTTTCC | |
Casp11 | Forward | ACAAACACCCTGACAAACCAC |
Reverse | CACTGAGTTCAGACTTGTTAAA | |
Il-1β | Forward | GCCACCTTTTGACAGTGATGAG |
Reverse | CCTGAAGCTCTTGTTGATGTGC | |
Il-18 | Forward | TCTGACATGGCAGCCATTGT |
Reverse | CAGGCCTGACATCTTCTGCAA | |
β-actin | Forward | GAGACCTTCAACACCCCAGC |
Reverse | ATGTCACGCACGATTTCCC |