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. 2022 Oct 18;144(43):19791–19798. doi: 10.1021/jacs.2c06599

Figure 6.

Figure 6

Temporal control of thrombin activity. (a) Scheme showing temporal control of thrombin activity regulated by an upstream delayed SDR mediated by RNase H. The output strand of this reaction is the G-quadruplex TBA that inhibits thrombin activity (dashed box). (b) Percentage of active thrombin VS time (from the addition of RNase H in solution) for different concentrations of RNase H. (c) Percentage of active thrombin after 3 h vs RNase H concentration. Experiments were performed in Tris HCl 20 mM, MgCl2 10 mM, EDTA 1 mM, 150 mM NaCl, pH 8.0 at T = 25 °C. [Target duplex] = 50 nM, [RNA-blocker] = 150 nM, [thrombin] = 0.5 nM, [fibrinogen] = 1 mg/mL.