FIGURE 1:

Propofol attenuates anterograde transport of axonal KIF5C vesicles. (A, B) Representative images of 7 DIV hippocampal neurons expressing Halo-KIF5C tail and visualized with JF549. Arrowheads indicate the axon. High-magnification images and kymographs show vesicles and axonal transport of Halo-KIF5C vesicles before and 10 min after treatment with (A) vehicle (DMSO) or (B) 10 µM propofol. For clarity, transport events from kymographs are redrawn as black lines. Kymograph lines with a positive slope indicate anterograde transport, and lines with a negative slope indicate retrograde transport. This convention is followed in all figures. Scale bar: 10 µm. (C, D) Histograms of axonal run lengths and velocities for axonal vesicles visualized with Halo-KIF5C tail. (E, F) Plots of the change in run length and velocity for each neuron after 10 min treatment with vehicle (DMSO) or propofol. DMSO: 27 cells; propofol: 25 cells.