TABLE 2.
Sequential i.m. and r. or i.m. immunization with partially purified C. difficile toxoid
| Group | Route (days) | Dosea (μg) of:
|
Adjuvant | No. of animals | |
|---|---|---|---|---|---|
| Toxin A | Toxin B | ||||
| A | i.m. (0) | 25 | 9 | Alum | 20 |
| r. (14, 21) | 25 | 9 | LT | ||
| B | i.m. (0) | 25 | 9 | Alum | 20 |
| r. (14, 21) | 25 | 9 | None | ||
| C | i.m. (0, 14, 21) | 25 | 9 | Alum | 15 |
| D | i.m. (0, 14, 21) | 25 | 9 | none | 15 |
| E | i.m. (0) | 0 | 0 | Alumb | 10 |
| r. (14, 21) | 0 | 0 | LTb | ||
| F | Weight control (unchallenged) | None | None | None | 5 |
a
Toxin A or B concentration determined by ELISA prior to formaldehyde inactivation.
b
Adjuvant administered in PBS.