Fig. 5.

Trem2 ablation does not impair CD3 T cell infiltration or MHC class II expression in lesions. SC sections from MHV–JHM-infected WT and Trem2^−/− mice were analyzed for CD3⁺ lymphocyte distribution and MHC class II expression. A, B Representative images of CD3 (green) and DAPI (blue) positive cells in demyelinated lesions. C Quantification of CD3 numbers per mm² of SCs form WT and Trem2^−/− mice at days 14 and 20 pi. D–E Representative images of MHCII (green) and DAPI (blue) staining in demyelinated lesions. Scale bar = 50 μm. F Quantification of the volume of MHCII positive cells per μm³ at days 14 and 20 pi. Data show the mean ± SEM from 12 to 18 lesions from 3 mice (4–6 lesions/mouse) per timepoint and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis followed by Bonferroni test