Fig. 8.

Trem2 ablation dysregulates expression of phagosome associated genes during MHV–JHM-induced demyelination. RNA from microglia (CD45^intCD11b⁺) and BMDM (CD45^hiCD11b⁺Ly6G⁻) from SCs of WT and Trem2^−/− mice at days 12 and 28 pi was subjected to nCounter analysis as depicted in Fig. 7. A, B Heatmaps show comparative expression of genes associated with inflammatory factors (A) and phagocytosis (B) in microglia and BMDM from WT and Trem2^−/− mice over time. C, D Normalized count value of Lpl, Cd36, Fn1, Itgax and Orl1 mRNA expression in microglia and BMDM from WT and Trem2^−/− mice over timepoint. Individual dots indicate cell populations from individual mice (for BMDM at day 12 pi) or from 2 to 3 pooled mice (for microglia and BMDM at days 0 and 28 pi). Data for show the mean ± SEM of each cell population from 4 individual or 2–3 pooled mouse per timepoint and are analyzed by unpaired two-tailed Student t test and two-way ANOVA analysis