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. 2022 Nov 4;24:249. doi: 10.1186/s13075-022-02938-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — NF-κB pathway mediated BD serum-treated M1-like macrophage polarization. A Representative western blot images (upper) and summary (lower) of NF-κB p65, phospho-p65 and IκBα of macrophages treated with BD (n=3) serum or HC (n=3) serum. Macrophages were pretreated with DHE and then were stimulated with BD serum for 48 h. B Representative histograms (left) and summary (right) of CD86 expression on DHE-treated and untreated macrophages stimulated with BD (n=6) serum and HC (n=6) serum. C TNF-α production by DHE-treated and untreated macrophages stimulated with BD serum (n=6) and HC (n=6) serum. Data were shown as mean±SD. *, p<0.05; **, p<0.01, ***, p<0.001, ****, p<0.001 by two-way ANOVA. M_BD, BD serum- treated macrophages; M_HC, HC serum- treated macrophages